Sekretariat Normal client4 2 2004-02-02T13:51:00Z 2004-02-02T15:33:00Z 2004-02-02T15:33:00Z 1 287 1638 Max von Pettenkofer-Institut 13 3 2011 9.2812

"The antiphlogistic strategy of Yersinia"

J. Heesemann and A. Sing,

Max von Pettenkofer-Institut, München

The physiological microbial flora of humans is efficiently controlled by the innate immune system. Microbial agents which are endowed with the capability to subvert, evade or get under control the innate immune response are defined as pathogens. Yersinia enterocolitca and Y. pestis evolved short-distance (sd) and long-distance (ld) weapons to attenuate the primary inflammatory response. The type III protein secretion system acts as sd-weapon by injection of effector proteins (Yops) into contacted cells. YopE, YopH, YopT and YopP downregulate production of proinflammatory cytokines and reactive oxygen. The long known V-antigen (LcrV) is secreted by yersinia and sensed by toll-like receptor 2 (TLR2) of macrophages leading to IL-10 induction and suppression of TNF-a. Preliminary studies revealed that TLR-2 does not only function as LcrV-signalling surface receptor but also translocates LcrV across the cytoplasma membrane to intracellular compartments where LcrV meets the two cytoplasmic microdetectors Nod1 and Nod2. Thus, TLR2 functions as signalling and shuttle molecule for LcrV. Strikingly LcrV-sensing by Nod1/2 also results in IL-10 production.

In conclusion, Yersinia is capable to modulate the innate immune response of the host in the sense of avoidance of a strong inflammatory response.

Sing A., Rost D., Tvardovskaia N., Roggenkamp A., Wiedemann A., Kirschning C. J., Aepfelbacher M., Heesemann J. 2002.

Yersinia V-Antigen Exploits Toll-like Receptor 2 and CD14 for Interleukin 10-mediated Immunosuppression.

J. Exp. Med. 196: 1017-1024.

Sing A, Roggenkamp A, Geiger AM, Heesemann J. 2002.

Yersinia enterocolitica Evasion of the Host Innate Immune Response by V Antigen-Induced IL-10 Production of Macrophages Is Abrogated in IL-10-Deficient Mice.

J Immunol. 168(3):1315-21